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collagen type iii alpha 1 chain col3a1  (Proteintech)


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    Proteintech collagen type iii alpha 1 chain col3a1
    Collagen Type Iii Alpha 1 Chain Col3a1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 791 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/collagen+type+iii+alpha+1+chain+col3a1/Collagen+Type+I+Antibody/pm41839308-88-36-43
    Average 96 stars, based on 791 article reviews
    collagen type iii alpha 1 chain col3a1 - by Bioz Stars, 2026-10
    96/100 stars

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    other:

    Article Title: Ketogenic diet induces autophagy to alleviate bleomycin-induced pulmonary fibrosis in murine models.
    Article Snippet: Aim of the Study: Ketogenic diet (KD) has been identified as an effective strategy in treating multiple diseases.. KD is capable of inducing autophagy which is an important therapeutic target for pulmonary fibrosis (PF).. This study aimed to investigate the effect of KD treatment on PF progression.

    Incubation:

    Article Title: FOSL1 Mediates Collagen Synthesis and Myofibroblast Transformation in Aortic Fibroblasts Under Ang II-Induced Aortic Dissection Conditions.
    Article Snippet: .. Sections were 6 incubated overnight at 4 °C with primary antibodies against FOSL1 (Santa Cruz Biotechnology, sc-28310, 1:100), matrix metalloproteinase 9 (MMP9) (Proteintech, 10375-2-AP, 1:200), collagen type I alpha 2 chain (COL1A2) (Proteintech, 66761-1-Ig, 1:200) and collagen type III alpha 1 chain (COL3A1) (Proteintech, 22734-1-Ig, 1:1000)]. ..



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    Fig. 2. Silencing of DLX2 governs the proliferation and apoptosis of HA-VSMC. (A) The silencing efficiency of shRNAs targeting DLX2 by RT-qPCR (n = 3). (B) The proliferation of HA-VSMC was examined using EdU (n = 3). (C) Detection of apoptosis of HA-VSMC by flow cytometry (n = 3). (D) Detection of COL1A1, COL3A1, MMP2 and MMP9 in HA-VSMC by western blot (n = 3). *p < 0.05 vs. the sh-NC group. The measurement data were expressed as mean ± SD. Unpaired t-test was performed for comparisons between two groups, one-way or two-way ANOVA was used for comparisons among multiple groups, and Tukey’s post hoc test was applied for pairwise comparisons. sh-NC, short hairpin RNA (shRNA) targeting negative control; sh-DLX2, short hairpin RNA (shRNA) targeting DLX2.

    Journal: The Tohoku Journal of Experimental Medicine

    Article Title: JMJD3 is Involved in Intracranial Aneurysm Development by Regulating DLX2 Expression through H3K27me3 Modification

    doi: 10.1620/tjem.2023.j048

    Figure Lengend Snippet: Fig. 2. Silencing of DLX2 governs the proliferation and apoptosis of HA-VSMC. (A) The silencing efficiency of shRNAs targeting DLX2 by RT-qPCR (n = 3). (B) The proliferation of HA-VSMC was examined using EdU (n = 3). (C) Detection of apoptosis of HA-VSMC by flow cytometry (n = 3). (D) Detection of COL1A1, COL3A1, MMP2 and MMP9 in HA-VSMC by western blot (n = 3). *p < 0.05 vs. the sh-NC group. The measurement data were expressed as mean ± SD. Unpaired t-test was performed for comparisons between two groups, one-way or two-way ANOVA was used for comparisons among multiple groups, and Tukey’s post hoc test was applied for pairwise comparisons. sh-NC, short hairpin RNA (shRNA) targeting negative control; sh-DLX2, short hairpin RNA (shRNA) targeting DLX2.

    Article Snippet: The membranes were blocked at room temperature with Tris-buffered saline-Tween 20 containing 5% bovine serum albumin (BSA) and incubated with diluted primary antibodies against rabbit antibodies to DLX2 (ab272902, Abcam), JMJD3 (ab169197, Abcam), collagen type I alpha 1 chain (COL1A1) (#91144, Cell Signaling Technologies, Beverly, MA, USA), collagen type III alpha 1 chain (COL3A1) (sc-271249, Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), matrix metalloproteinase-2 (MMP-2) (ab181286, Abcam), matrix metalloproteinase-9 (MMP9) (ab76003, Abcam), H3K27me3 (#9733, Cell Signaling Technologies), and GAPDH (ab9485, Abcam) overnight at 4°C and with the secondary antibody goat anti-rabbit IgG antibody (ab6721, Abcam) or rabbit anti-mouse IgG antibody (ab6728, Abcam) at room temperature.

    Techniques: Quantitative RT-PCR, Flow Cytometry, Western Blot, shRNA, Negative Control

    Fig. 5. Silencing of JMJD3 inhibits the formation of intracranial aneurysm. Rats with intracranial aneurysm were administered with sh-DLX2 or sh-JMJD3 alone, or with oe-DLX2. Model, successfully modeled intracranial aneurysm rats. (A) Detection of COL1A1, COL3A1, DLX2, and JMJD3 in the artery of intracranial aneurysm rat by western blot (n = 8). (B) The blood pressure of rats in each group (n = 8). (C) Histopathological changes of arteries in rats with intracranial aneurysm detected by HE staining (n = 8). (D) The levels of inflammatory factors TNF-α, IL-1β, and IL-6 in the tail vein blood of rats by ELISA (n = 8). *p < 0.05 vs. the sham group; #p < 0.05 vs. the sh-NC group; &p < 0.05 vs. the sh-JMJD3 + oe-NC group. The measurement data were expressed as mean ± standard deviation. Two-way ANOVA was used for comparisons among multiple groups and Tukey’s post hoc test was used for pairwise comparisons.

    Journal: The Tohoku Journal of Experimental Medicine

    Article Title: JMJD3 is Involved in Intracranial Aneurysm Development by Regulating DLX2 Expression through H3K27me3 Modification

    doi: 10.1620/tjem.2023.j048

    Figure Lengend Snippet: Fig. 5. Silencing of JMJD3 inhibits the formation of intracranial aneurysm. Rats with intracranial aneurysm were administered with sh-DLX2 or sh-JMJD3 alone, or with oe-DLX2. Model, successfully modeled intracranial aneurysm rats. (A) Detection of COL1A1, COL3A1, DLX2, and JMJD3 in the artery of intracranial aneurysm rat by western blot (n = 8). (B) The blood pressure of rats in each group (n = 8). (C) Histopathological changes of arteries in rats with intracranial aneurysm detected by HE staining (n = 8). (D) The levels of inflammatory factors TNF-α, IL-1β, and IL-6 in the tail vein blood of rats by ELISA (n = 8). *p < 0.05 vs. the sham group; #p < 0.05 vs. the sh-NC group; &p < 0.05 vs. the sh-JMJD3 + oe-NC group. The measurement data were expressed as mean ± standard deviation. Two-way ANOVA was used for comparisons among multiple groups and Tukey’s post hoc test was used for pairwise comparisons.

    Article Snippet: The membranes were blocked at room temperature with Tris-buffered saline-Tween 20 containing 5% bovine serum albumin (BSA) and incubated with diluted primary antibodies against rabbit antibodies to DLX2 (ab272902, Abcam), JMJD3 (ab169197, Abcam), collagen type I alpha 1 chain (COL1A1) (#91144, Cell Signaling Technologies, Beverly, MA, USA), collagen type III alpha 1 chain (COL3A1) (sc-271249, Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), matrix metalloproteinase-2 (MMP-2) (ab181286, Abcam), matrix metalloproteinase-9 (MMP9) (ab76003, Abcam), H3K27me3 (#9733, Cell Signaling Technologies), and GAPDH (ab9485, Abcam) overnight at 4°C and with the secondary antibody goat anti-rabbit IgG antibody (ab6721, Abcam) or rabbit anti-mouse IgG antibody (ab6728, Abcam) at room temperature.

    Techniques: Western Blot, Staining, Enzyme-linked Immunosorbent Assay, Standard Deviation

    Primer sequences used for qRT-PCR analysis

    Journal: BMC Cancer

    Article Title: Nitric-Oxide Synthase trafficking inducer (NOSTRIN) is an emerging negative regulator of colon cancer progression

    doi: 10.1186/s12885-022-09670-6

    Figure Lengend Snippet: Primer sequences used for qRT-PCR analysis

    Article Snippet: Those procured from Santa Cruz Biotechnology, USA were used at 1:250 dilution, which included anti-Collagen type III Alpha 1 chain/COL3A1 (Cat no. sc-514601), anti-Regulator of G-protein signalling 2/RGS2 (Cat no. sc-100761) and anti-Occludin/OCLN (Cat no. sc-133256). anti-Desmoplakin/DSP (Cat no. A303-355A) and anti-Junctional adhesion molecule A/F11R (Cat no. A302-891A) antibodies were from Bethyl lab, USA.

    Techniques: Sequencing

    NOSTRIN-induced alteration of transcripts associated with epithelial mesenchymal transition in HCT116 cells

    Journal: BMC Cancer

    Article Title: Nitric-Oxide Synthase trafficking inducer (NOSTRIN) is an emerging negative regulator of colon cancer progression

    doi: 10.1186/s12885-022-09670-6

    Figure Lengend Snippet: NOSTRIN-induced alteration of transcripts associated with epithelial mesenchymal transition in HCT116 cells

    Article Snippet: Those procured from Santa Cruz Biotechnology, USA were used at 1:250 dilution, which included anti-Collagen type III Alpha 1 chain/COL3A1 (Cat no. sc-514601), anti-Regulator of G-protein signalling 2/RGS2 (Cat no. sc-100761) and anti-Occludin/OCLN (Cat no. sc-133256). anti-Desmoplakin/DSP (Cat no. A303-355A) and anti-Junctional adhesion molecule A/F11R (Cat no. A302-891A) antibodies were from Bethyl lab, USA.

    Techniques: Binding Assay